GOT-AST: programming in a semi-auto biochemistry analyzer


Glutamate Oxaloacetate Transaminase (aspartate aminotransferase) is an enzyme that is mostly present in the liver cells, kidney, skeletal system, and heart muscles. An increase in the value of GOT-AST in the blood mainly indicates that there is damage to those major organs. Similarly, a lower level of GOT-AST can be due to pregnancy, diabetic ketoacidosis, etc.

Everything that I state below will be based on a semi-auto biochemistry analyzer and can differ from one manufacturer to another.

Principle reaction

AST enzyme converts the 2-oxoglutarate, and L-aspartate to L-glutamate and oxaloacetate. The oxaloacetate reacts with NADH in the presence of malate dehydrogenase (MDH) to form NAD. The rate of degradation of NADH to NAD causes a decrease in the absorbance of the light. This is proportional to the activity of the GOT-AST enzyme.


Normally, you can store both the reagents (R1 and R2) at 2-80C  till the expiration date. But, once you open the bottle, the lifespan will reduce to about 28 days (at 2-80C ) for R1 and 90 days for R2. Also,  for a working solution, the lifespan will be about  14 days.


  • Wear an apron and surgical gloves before carrying out the measurement.
  • Look for the expiry date of the reagents during purchase and measurement time. Suppliers tend to give you reagent kits with a low expiry interval.
  • Bring the reagents and samples to room temperature before you can carry out any measurement.
  • Always store reagents in the refrigerator when not in use.

Sample preparation

To prepare the working solution, mix R1 and R2 reagents in a ratio of 5:1. After that, add 100 μl of sample to 1000 μl of the prepared working solution. Finally, feed the solution into a semi-auto biochemistry analyzer and get the result.

If the activity of the GOT-AST enzyme is very high (out of range), you need to dilute it with 0.9% NaCl or distilled water in a ratio of 1: y. Then when you get the final result, multiply the value with a dilution factor (1+y).

You will get a decreasing curve in the biochemistry analyzer.

Programming for GOT-AST in a semi-auto biochemistry analyzer (IFCC method)

GOT-AST, semi-auto biochemistry analyzer

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